Phytochemical and Antimicrobial Studies on Vitex Chrysocarpa (Planch ex Benth.)

Chinyere Veronica Ilodibia, John Chimezie Okafor, Ebele Esther Akachukwu, Ngozi Anasthesia Igboabuchi, Uju Maureen Chukwuma, Nkechi Ruffina Adimonyemma

Abstract


Ethanolic leaf, stem and root extracts of Vitex chrysocarpa were investigated for the presence and composition of these phytochemicals (alkaloid, flavonoid, phenol, tannin, sterol, hydrogen cyanide, anthraquinone, saponin and terpenoid) using standard techniques. Their antimicrobial activities at different concentration against some selected clinical pathogens (bacterial strains Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa, Fungal strain Aspergilus niger ) were also studied using agar diffusion method. Minimum Inhibitory Concentration against the organisms was also evaluated. Analysis of Variance (ANOVA) was employed in data analysis. Qualitative and percent quantitative phytochemical results showed that Ethanolic leaf, stem and root extracts contained these phytochemicals assayed but at varied quantities. Antimicrobial studies indicated that the ethanolic leaf, root and stem extracts of Vitex chrysocarpa inhibited the growth of the pathogens but at varied levels and the inhibition was extracts concentration dependent. However, the extracts showed higher inhibition against the fungal strain than the bacterial strains. Inhibitory effect of the leaf extract was significantly higher than those of the stem and root extracts with minimum inhibitory concentration of 50mg/ml for S. aureus and E. coli, 25mg/ml for P.  aeruginosa and 12.50mg/ml for A. Niger. For the stem, the minimum inhibitory concentration is at 50mg/ml for S.  aureus and P.  aeruginosa , 75mg/ml for E.coli and 25mg/ml for A. Niger . For the root, minimum inhibitory concentration is at 75 mg/ml for S.  aureus and E.coli, 25mg/ml for P.  aeruginosa and 50mg/ml for A.niger.

Antibiotic had a better activity when compared to the extracts at the same concentration. The data obtained from this study showed that the plant possessed antimicrobial properties especially antifungal and could be used in the treatment of bacterial and fungal infections but more especially the latter.


Keywords


Vitex chrysocarpa, phytochemicals, antimicrobial, minimum inhibitory concentration.

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References


Ajoku G, Ibrahim K, Enwerem N. Antimicrobial activity of a Nigerian medicinal plant, Hibiscus sabdariffa. Nig. J Biotechnol. 2001;12(2):82-5.

Palombo EA. Phytochemicals from traditional medicinal plants used in the treatment of diarrhoea: modes of action and effects on intestinal function. Phytother Res. 2006;20(9):717-24. DOI: 10.1002/ptr.1907 PMID: 16619336

Sofowora A. Recent trends in research into African medicinal plants. J Ethnopharmacol. 1993;38(2-3):209-14. PMID: 8510470

Sokmen A, Sokmen M, Daferera D, Polissiou M, Candan F, Unlu M, et al. The in vitro antioxidant and antimicrobial activities of the essential oil and methanol extracts of Achillea biebersteini Afan. (Asteraceae). Phytother Res. 2004;18(6):451-6. DOI: 10.1002/ptr.1438 PMID: 15287068

Kunle OF, Egharevba HO. Preliminary studies on Vernonia ambigua: phytochemical and antimicrobial screening of the whole plant. Ethnobotanical Leaflets. 2009;2009(10):2.

Ilodibi CV, Ezeja IJ, Akachuk EE, Chukwum MU, Egboka TP, Emeka AN. Phytochemical Screening and Antimicrobial Effects of Aqueous and Ethanol Leaf and Stem Extracts of Gongronema latifolium Benth. Res J Botany. 2015;10(2):50-60. DOI: 10.3923/rjb.2015.50.60

Ganapaty S, Vidyadhar K, Ganga-Rao B. Antimicrobial Activity of two Vitex species. Ind J Nat Prod. 2005;21:46-9.

Harborne A. Phytochemical methods a guide to modern techniques of plant analysis. New York: Chapman and itzl Press; 1973.

Chemists AoOA, Chemists AoOA. Official Methods of Analysis. 15th ed. Washington DC., USA1990.

Kirk I, Sawyer R. Frait Pearson Chemical Analysis of Food. 8th ed. Edinburgh: Longman Scientific and Technical; 1998.

Esimone C, Adikwu M, Okonta J. Preliminary antimicrobial screening of the ethanolic extract from the lichen Usnea subfloridans (L). J Pharm Res Dev. 1998;3(2):99-102.

Osadebe P, Ukwueze S. A comparative study of the phytochemical and anti-microbial properties of the Eastern Nigerian specie of African Mistletoe (Loranthus micranthus) sourced from different host trees. Bio Res. 2004;2(1):18-23.

Ghoshal S, Prasad BN, Lakshmi V. Antiamoebic activity of Piper longum fruits against Entamoeba histolytica in vitro and in vivo. J Ethnopharmacol. 1996;50(3):167-70. PMID: 8691851

Iwu M, Duncan AR, Okunji CO. New antimicrobials of plant origin. Perspect New Crop New Uses. 1999;3(2):457-62.

Edeoga HO, Okwu DE, Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. African J Biotech. 2005;4(7):685-8. DOI: 10.5897/ajb2005.000-3127

Joshi B, Lekhak S, Sharma A. Antibacterial property of different medicinal plants: Ocimum sanctum, Cinnamomum zeylanicum, Xanthoxylum armatum and Origanum majorana. Kathmandu Univ J Sci Engin Technol. 2009;5(1):143-50.

Akiyama H. Antibacterial action of several tannins against Staphylococcus aureus. J Antimicrob Chemotherap. 2001;48(4):487-91. DOI: 10.1093/jac/48.4.487

Ogu G, Aisuodionoe M, Nwachukwu P. Antiplasmodial activity of Caesalpinia Pulcherrima (Swarts) stem. Int J Bio Pharma Allied Sci. 2012;1(2):168-78.

Dahanukar S, Kulkarni R, Rege N. Pharmacology of medicinal plants and natural products. Indian J Pharmacol. 2000;32(4):S81-S118.

Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev. 1999;12(4):564-82. PMID: 10515903

Hassan H, Sule M, Usman M, Ibrahim A. Preliminary phytochemical and antimicrobial screening of they stem bark extracts of Bauhinia rufescence Lam using some selected pathogens. Bayero J Pure Appl Sci. 2009;2(2):53-5.




DOI: http://dx.doi.org/10.21859/ajlsr-040403

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