Assessing of salt effect in regulation of growth and antioxidant defence in Arthrocnemum indicum under Arsenic stress alone or with salinity

Dhouha Belhadj SGHAIER

Abstract


Metal pollution is one of the most acute environmental issues, aggravated by human activities. In the present study, authors intend to determine the tolerance traits of Arthrocnemum indicum to avoid As toxicity. In order to evaluate the mechanisms responsible for Arsenic (As) tolerance, plants were grown in semi-controlled conditions upon exposure to different As concentrations (0, 200, 500 and 800 µM) supplemented or not with NaCl (0, 200 mM). Growth parameters, micronutrient uptake, proline and glycine betaine contents and antioxidant enzymes activities (SOD, GPX and APX) were assessed. Plants demonstrated a good growth even after prolonged exposure to high metal concentrations. On the other hand, the GB and malondialdehyde content in the leaves of stressed plants increased significantly in all treatments. However, proline showed a steady level. Activities of SOD, GPX and APX varied according to the applied stress. Our results indicated that As induced oxidative stress in Arthrocnemum indicum and enzymatic and non-enzymatic antioxidants played significant roles in tolerance to As toxicity.

Keywords


Arsenic, combined stress, mineral uptake, antioxidant mechanisms

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